quantitative synthetic monkeypox virus dna  (ATCC)

Journal: bioRxiv

Article Title: Probe-Free Multiplexed RPA Detection Via Single-Molecule Nanopore Sensing and Deep Learning Classification

doi: 10.1101/2025.04.24.650542

Figure Lengend Snippet: (a) The selected genes for Monkeypox (Mpox) and human RNase P DNA serve as process controls. The multiplex assay includes forward primers (F 1 , F 2 ) and reverse primers (R 1 , R 2 ). (b) Oligonucleotide sequences of both primer sets. (c) Gel electrophoresis validation of the multiplex assay, where the first two lanes correspond to T 1 , the next two to T 2 , followed by two lanes representing the multiplex assay (T 1 + T 2 ), and the last two lanes containing the NTC samples.

Article Snippet: Synthetic Mpox DNA (Quantitative Synthetic Monkeypox virus DNA, VR-3270SDTM, 100 μL, 2.8 × 105 copies/μL) was obtained from ATCC Bio, while Human RNase P DNA (Hs_RPP30 Positive Control, #10006626) was sourced from IDT.

Techniques: Multiplex Assay, Nucleic Acid Electrophoresis, Biomarker Discovery

Journal: One Health

Article Title: Bioinformatics-based screening tool identifies a wide variety of human and zoonotic viruses in Trujillo-Peru wastewater

doi: 10.1016/j.onehlt.2024.100756

Figure Lengend Snippet: Confirmation of selected human viruses (SARS-CoV-2 [N1 and N2 genes] and monkeypox viruses) in wastewater collected from Trujillo-Peru by digital droplet PCR.

Article Snippet: Monkeypox viral DNA was purchased from ATCC (ATCC Number: VR-3270SD).

Techniques: